In this study, a heart rate variability (HRV) analysis guided by respiration is conducted to assess changes in autonomic nervous system (ANS) regulation during pro- nounced heat stress induced by repetitive exposures to dry sauna in 13 young healthy subjects. The spectral power at the low frequency (LF) band [0.04, 0.15] Hz and at the high frequency band centered around the mean respiratory rate, estimated from ECG-derived respiration signals, are studied. The mean heart rate (HR), the total power of HRV, and the normalized LF power are also analyzed. Results show that mean HR and normalized LF power increase significantly after basal stage, not only during the sauna sessions, but also at the intermediate short rest stages and recovery phase after 30 min. A significant re- duction in the total power of HRV during all sauna ses- sions is observed, while an increased power in LF band is shown only during recovery which might be related to im- proved cardiac function (increased cardiac output and re- duced peripheral vascular resistance) after heat exposure. Respiratory rate does not change significantly during the protocol but a negative trend at stress stages may indicate a reverse reaction against an over-activation of the sympa- thetic branch. In conclusion, exposure to heat stress shifts sympathovagal balance of ANS toward sympathetic domi- nance that increases HR and diminishes HRV.