Aims: Optical mapping (OM) has been a useful tool in the discovery of many arrhythmic mechanisms; furthermore, advances in camera sensors over the past decade have made the setup of an OM system very affordable, with the price of OM cameras having dropped from $60K to under $3K. Given the widespread use of OM systems, it is desirable to have programs that can both analyze and display data interactively and independently of the operating system.
Results: We developed a Unified Electrophysiology Mapping Framework (Unimapper) to facilitate the analysis, visualization and exchange of electrophysiology data. The program inputs experimental (and simulation) data and processes the data (e.g. filtering, de-drifting, smoothing, Hilbert’s transform, etc). It can display the data in time with variable speed; it can also display voltage and/or calcium signals from dual optical recordings and can display the voltage and calcium signals in time for any chosen pixel and plot phase-space portraits of V-Ca. Line-scan figures along any direction of the tissue are also displayed simultaneously. Examples shown include V-Ca OM from pig, rabbit, guinea pig hearts and monolayers. Dynamics include SA-node pacing, electrode pacing, concordant-alternans, discordant-alternans, reentrant spiral waves and fibrillation.
Conclusions: We present an interactive program that analyzes and inter-actively displays OM experimental data in a web browser, independent of the device used. Data can be visualized in various modes and analyzed images, V(t)/Ca(t) signals, alternans and other measurements can be exported and saved. We expect that this software will facilitate creation of a repository of experimental data for modelers and other researchers.