In a previous study we proved that our proposed indexes of respiratory sinus arrhythmia (RSA) sensitivity (RSAS), frequency (RSAFC) and power (RSAPC) coupling better elucidated the mechanism of chirped breathing effects on RSA. Now, in 25 healthy subjects, we compared the effects provoked by combining continuously increasing and then decreasing tidal volume (VT, 0.5-2.5 l) with chirped respiratory frequency (RF, 0.15-0.5 Hz) versus those of the same chirped RF at fixed VT (0.8 l) on the instantaneous 45-s time-courses of central frequencies and powers of high-frequency components (CFHF, PHF) of RR intervals (CFHFRR and PHFRR) and respiration (CFHFRES and PHFRES), estimated by a time-frequency distribution. We used the CFHFRES-CFHFRR relation and their difference (ΔCFHF) as RSAFC indexes, PHFRR/PHFRES coherence (RSACO) as RSAPC index, and √(PHFRR/PHFRes) as RSAS. The combined VT-RF maneuver provoked, in relation to the chirped RF maneuver: 1) accentuation of the progressive reduction of RSAS dynamics, shown by their smaller (p<0.04) mean values at 7.5, 15, 22.5 and 30s; 2) smaller (p<0.04) slope and intercept of the CFHFRES-RSAS relation; 3) smaller (p<0.04) 15, 22.5 and 30s means of RSACO dynamics; 4) greater (p<0.001) 7.5,15 and 22.5s means of CFHFRR dynamics; 5) greater (p<0.02) intercept and smaller (p<0.04) slope of the CFHFRES-CFHFRR relation; 6) smaller (p<0.04) 15 and 22.5s means and larger (p<0.002) 7.5 and 30s means of ΔCFHF dynamics. Our findings support that 0.5-2.5 l increasing-decreasing VT provokes an important depression of RSAS and RSAPC measures in the entire RF range, and opposite successive effects on RSAFC indexes: from RF of 0.15 to 0.42 Hz, VT variations enhances them and from 0.42-0.5 Hz, VT changes reduces them. Thus, the use of our instantaneous RSA spectral measures clarifies the functional explanation of the effects produced by the continuously increasing-decreasing VT maneuver on the RSA mechanism time course.